A nurse is reinforcing teaching about the iPLEDGE program with a female client who has a new prescription for isotretinoin. The nurse should tell the client that which of the following is a requirement of the program?

Clients must have a Papanicolaou test every 6 months during treatment.

Female clients must have a negative mammogram prior to beginning therapy.

Clients must begin a daily supplement of vitamin A for 1 month prior to initiating therapy

Sexually active female clients must use two forms of birth control during treatment